Immunofluorescence

Immunofluorescence assays utilize the light from a fluorescence microscope to detect influenza virus antigens. The fluorescence microscope provides results in approximately two to four hours with increased sensitivity and specificity. In the assay, both indirect fluorescent antibody (IFA) and direct fluorescent antibody (DFA) appear to aid in the detection of influenza A and B antigens in respiratory tract specimens. The fluorescent microscope is insufficient to identify and subtype influenza A and influenza B viruses.